| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Fremont Soccer Club FSC United Gold 2016 (S) |
04054-0013B8U-0251 | Kelly Skinner |
| A2 | Omaha United Soccer Club Omaha United SC 2016B White |
03133-0012B8U-0059 | Oscar Castro |
| A3 | Gretna Elite Academy GEA Academy White II 16B |
02077-0013B8U-0137 | Oumar Sissoko |
| A4 | Norfolk Football Club NFC 2016B |
04057-0013B8U-0009 | Mark Konrady |
| A5 | Omaha Legends Omaha Legends - Jr.Academy B8U |
03089-0013B8U-0070 | Collin Hinds |
| A6 | Sting Soccer Sting NE 16B Dotson |
03137-0012B8U-0123 | Kris Dotson |
| A7 | Sporting Nebraska FC Sporting Nebr B LNK Acad 16 |
03036-0013B8U-2323 | Edward Meitzen |
| A8 | United Association Football UAF 2016/2017B Blue |
03001-0013B8U-0024 | Ian McBride |
| A9 | Gretna Elite Academy GEA Academy White I 16B |
02077-0013B8U-0131 | Oumar Sissoko |