| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Iowa - Council Bluffs Soccer Club CBSC U12B Black |
0O002-0012B2U-0008 | Conner James |
| A2 | Omaha United Soccer Club Omaha United SC 2012B White |
03133-0012B2U-0048 | Cesar Garcia |
| A3 | Sting Soccer Sting NE 12B Davis |
03137-0012B2U-0087 | Reed Davis |
| A4 | Elkhorn Athletic Association Nebraska Roots 2012B III |
03004-0012B2U-1361 | Lane Peercy |
| A5 | Gretna Elite Academy GEA White 12B |
02077-0013B2U-0082 | Ariel Ayala-Reyes |
| A6 | Sting Soccer Sting NE 12B Dotson |
03137-0013B2U-0120 | Kris Dotson |
| A7 | United Association Football UAF 2012B Gray |
03001-0012B2U-0023 | Daniel Douglas |
| A8 | Bennington Soccer Club BENSC Force 12B (S) |
03057-0012B2U-0077 | Gina Lindsey |
| A9 | Beatrice Nighthawks Beatrice Nighthawks - B3 |
03060-0013B2U-0098 | Manalle Armstrong |
| A10 | Dreamers FC DFC Aztecs 12 Blue |
03134-0013B2U-1805 | Greg Milius |
| A11 | Lincoln Spirit Soccer Club LSC Arsenal |
03024-0012B2U-3708 | Lee Dreyer |
| A12 | Lincoln Spirit Soccer Club LSC Chelsea FC |
03024-0012B2U-3712 | Eric Ojeda |