| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Ralston Soccer Club Ralston U12- Boys |
03023-0013B2U-0109 | Taylor Kroh |
| A2 | Bellevue Soccer Club BSC Pumas - Spring 24 |
02039-0013B2U-0046 | Nicholas Crampton |
| A3 | Iowa - Glenwood Soccer Club GLN Fusion (S) |
0O003-0012B2U-0015 | Richard Billingsley |
| A4 | Plattsmouth Soccer Club PLA 12UB Outlaws |
02040-0013B2U-0037 | Troy Nelson |
| A5 | Sporting Nebraska FC Sporting Nebr Vipers 12 |
03036-0013B2U-2375 | Jonnathan Pinzon |
| A6 | Sporting Nebraska FC Sporting Nebraska FC Crew 12 |
03036-0013B2U-2353 | William Lamme |
| A7 | Nebraska City Soccer Club NCYSC Burn |
06080-0013B2U-0112 | Adolfo Loarca |
| B1 | Blair Soccer Club Blair FC Arsenal |
04019-0012B2U-1086 | Justin Boerma |
| B2 | Elkhorn Athletic Association EAA Excelsior |
03004-0013B2U-1383 | Christopher Fassero |
| B3 | Nebraska City Soccer Club NCYSC Inferno |
06080-0013B2U-0008 | Monica Cavanzon |
| B4 | Papillion Soccer Club PSC U12B Tornadoes (S) |
02016-0013B2U-0083 | Warren Polensky |
| B5 | Sporting Nebraska FC Sporting Nebraska FC Intensity 12 |
03036-0013B2U-2383 | Zachary Johnston |
| B6 | Sporting Nebraska FC Sporting Nebraska FC Stampede 12 |
03036-0013B2U-2359 | Tammy Jung |
| B7 | Syracuse Soccer Club Syracuse Rockets B12U |
03086-0013B2U-0006 | Joe Reisdorff |